Just half an hour before the final whistle of the match between Roma and Juventus former Real Madrid striker Cristiano Ronaldo had an altercation with Giallorossi skipper Alessandro Florenzi during the Serie A 2019 tie at Stadio Olimpico.
It was Alessandro Florenzi and Edin Dzeko who scored the goals of the match in the 79th and 92nd minute.
The top four from Serie A qualify for next season's Champions League.
At the end of a week that saw Juve boss Massimiliano Allegri forced to deny rumours he was leaving, his side let in late goals to suffer only their third league defeat of the season.
The removal of stripes on home kit is the first time in over a century since the club was established in 1897, although Juventus players have worn striped kits of various types in history.
"A great (goalkeeper Antonio) Mirante kept us up in the first half but I am satisfied with the game", said Roma coach Claudio Ranieri.
AC Milan is above Roma thanks to a better head-to-head record, which is the tiebreaker in Italy.
They occupy sixth place with 62 points on the board, level with AC Milan and just one point off fourth place Inter Milan.
"We must continue to believe in it".
After Florenzi missed the target again, Dzeko stepped up to make sure of the points in the second minute of stoppage time.
Fabio Quagliarella pulled a goal back for Sampdoria converting a penalty in injury time to take his Serie A leading tally to 26 goals - five more than Juventus star Ronaldo.
Belotti nearly scored after the break but Sassuolo goalkeeper Andrea Consigli denied his overhead kick.