Another activist glued himself to the side of the train.
The stunt comes on the third day of a series of climate change protests which have led to almost 300 people being arrested in the capital.
Climate group Extinction Rebellion has ratcheted up its protests in recent weeks, blocking Marble Arch, Oxford Circus and Waterloo Bridge, smashing a door at the Shell building and shocking lawmakers with a semi-nude protest in parliament.
Extinction Rebellion spokesman James Fox said the group had attempted to maintain a blockade overnight at four sites in central London before the police came to impose the new restriction.
"The safety of our customers and staff is our number one priority and we're already working closely with the police to manage the impact on London's transport network".
Transport for London (TfL) said: "We will restore access as soon as we are able to do so". "We so far have 55 bus routes closed and 500,000 people affected as a result ... we are satisfied that this threshold has been met and this course of action is necessary".
London Mayor Sadiq Khan said in a statement that while he's sympathetic to the environmental movement, the effort to disrupt public transportation is counter-productive.
The police have ordered the protesters to confine themselves to a zone within Marble Arch, a space at the junction of Hyde Park, the Oxford Street main shopping thoroughfare and the Park Lane street of plush hotels.
A Transport for London spokesman said it was aware of plans for demonstrations on the Underground and urged people to check for travel updates before setting off.
In order to attract attention, the protesters are deliberately trying to get themselves arrested - more than 200 arrests have been made during the demonstrations in the last three days.
"Targeting public transport in this way would only damage the cause of all of us who want to tackle climate change", he said in a statement.
Five people were arrested on suspicion of criminal damage following disruption outside commercial premises in Lambeth on Monday and have since been released under investigation. We speak to Clare Farrell, one of the co-founders of the environmental action group Extinction Rebellion, and Farhana Yamin, global environmental lawyer who helped draft the landmark 2015 Paris Agreement.
Elsewhere on Tuesday, 29 people were arrested after hundreds of activists from the Extinction Rebellion Scotland group occupied Edinburgh's North Bridge, bringing traffic to a standstill.