However, Elon Musk's commercial space flight company successfully completed its first mission for the USA military on Sunday.
This next-generation Global Positioning System satellite is three times more accurate than previous versions and eight times better at anti-jamming, Heather Wilson, secretary of the Air Force says.
Although there wasn't enough propellant for a landing, the rocket's first stage was successful with the single engine powering the Falcon 9's second stage ignited as planned.
It was SpaceX's fifth attempt following technical and weather delays.
GPS III SV01 is the first of an entirely new, next generation GPS satellite created to modernize the GPS constellation. One of its Falcon 9 rockets took the Air Force's new GPS III satellite into orbit.
Vespucci's nickname honors Italian cartographer and explorer Amerigo Vespucci, after whom North and South America were named.
The US Air Force monitors the location of the launch known as the 45th Space Wing, helping to set up road blocks and clear relevant air space.
The next GPS III satellite is due to launch in mid-2019, Mr Eschenfelder said, while subsequent satellites undergo testing in the company's Colorado processing facility.
GPS III's new L1C civil signal also will make it the first GPS satellite broadcasting a compatible signal with other worldwide global navigation satellite systems, like Galileo, improving connectivity for civilian users. This is the 20th major rocket launch from Florida this year. However, he added that future GPS III missions may feature attempts to recover the first stage, depending on flight results from Sunday's mission. In September 2017, the Air Force declared the satellite "Available for Launch" (AFL) and had the company place it into storage.
It was built to be the first of 32 in production by Lockheed under contracts worth a combined $US12.6 billion for the Air Force GPS III program, according to Lockheed spokesman Chip Eschenfelder.