The mission lifted off at 10:16 am PST (01:16 p.m. EST), from Space Launch Complex 40 (SLC-40) at Cape Canaveral Air Force Station.
SpaceX has enjoyed notable success landing its Falcon 9 rocket, which enables the company to reuse the vehicle for decreased costs, among other things.
Private spaceflight company SpaceX launched its uncrewed Dragon Cargo Ship on Wednesday for International Space Station (ISS), but unfortunately, he failed to land the rocket successfully.
The launch marked the fourth flight of the Hawthorne-based aerospace firm's $62 million reusable Falcon 9 first-stage rocket booster, which reportedly failed to land properly because of hydraulics issues.
But the tall portion of the rocket missed its goal of securing an upright landing on the solid ground at Cape Canaveral's Landing Zone 1.
The first stage rocket had separated normally several minutes after the lift-off, but as it approached the landing site, it started spinning rapidly.
"Appears to be undamaged and is transmitting data". Musk also shared the video of a rocket landing on his Twitter account.
Just like the Falcon 9 booster used on the SmallSat mission, the Dragon spacecraft was used in missions before.
Luckily, the mission controllers were able to stabilize the rocket in time with some bursts from the engine, bringing the first stage in for a soft landing on water off the coast of Florida. The mice and their habitat were loaded into spacecraft shortly before launch, but the spoiled food had to be replaced ahead of launch.
The capsule is due to arrive at the space station on Saturday morning.