The long-awaited trial of Guzman, who prosecutors believe hired assassins to knock off rivals with a string of accused violent acts to protect the cartel, will start with the jury pool arriving answering a lengthy questionnaire, according to the New York Post.
Federal authorities have imposed high security to prevent Guzman from slipping away yet again.
That led to speculation Monday that Guzman would be held in a special cell deep within the Brooklyn courthouse facility.
At pretrial hearings leading to the trial, heavily armed federal officers and bomb-sniffing dogs patrolled outside.
And for further protection- the jurors names will be withheld from the public.
Opening arguments are scheduled to start on November 13, after a week set aside for jury selection, held behind closed doors with only a select "pool" of reporters admitted under the tightest security reserved for the most risky defendants.
The trial in Brooklyn under tight security brings 17 criminal charges against the 61-year-old former head of the Sinaloa drug cartel, including drug trafficking, money laundering and running a criminal organization.
USA prosecutors contend that from 1989 to 2014, the cartel smuggled at least 340,892 pounds (154,626 kg) of cocaine into the United States, as well as heroin, methamphetamine and marijuana, raking in $14 billion.
Legal experts say prosecutors have a rock-hard case against Guzman. "I think this is indicative of the fact that the Sinaloa cartel has probably infiltrated the drug industry in our country", said PNP spokesman Senior Supt. Gómez said a journey to the rugged Sierra Madre by American actor Sean Penn put investigators on Guzman's doorstep.
He was captured in Guatemala in 1993 and extradited to Mexico. After a 2001 escape, he kept the machine moving while on the run, hiding for 13 years in plain sight. He was recaptured in Mexico in February 2014. He founded the Sinaloa Cartel in 1980. "Probably not. The machine keeps rolling", says Rob Heroy, a North Carolina lawyer who has defended other Mexican drug barons. In the United States, opioid addiction has spiralled into an epidemic. Guzman sat at the defense table listening through an interpreter and wearing street clothes - a dark suit and a white shirt with an open collar - instead a jail uniform for the first time since he was extradicted to the United States early previous year. "Guzman Loera made billions of illicit dollars". US Marshals will escort them every day.
Bacon reported from Virginia.